Session Overview |
Biophotonics - Bloc 1Room: International 2 |
Date: Wednesday, June 06 |
13:15 |
Transient absorption microscopy hemes: contrast mechanisms and potential applications
Main Author: Jesse Wilson Organization: Colorado State University, United States Heme proteins are key players in electron transport, oxygen storage and transport, and catalysis. Hemes are mosty non-fluorescent, but have interesting ultrafast transient absorption responses that can be leveraged for imaging contrast. Potential applications include noninvasive biopsy for mitochondrial and metabolic disorders, and studying tumor microenvironment and therapy response. |
13:40 |
Quantitative Chemical Imaging of Cells and Tissue with BCARS microscopy
Main Author: Charlie Camp Organization: NIST, United States The ability to noninvasively image the dynamic chemical composition within cells and tissues would revolutionize our understanding of biology and disease. Molecular vibrational imaging techniques detect the small oscillations between bonded atoms, providing dense spectral information about composition and state without the addition of fluorophores or dyes. Technologies, such as Raman and infrared microscopies, have offered this capability for over half a century, but significant limitations in speed, resolution, or sample preparation have prevented their ubiquity. We have developed a broadband coherent anti-Stokes Raman scattering (BCARS) microscopy platform with an unprecedented combination of speed, sensitivity, and spectral breadth, enabling chemical hyperspectral imagery in minutes rather than hours. Additionally, this method generates a built-in reference signal at each pixel; thus, enabling pixel-by-pixel direct comparison of results between samples and microscope platforms. Demonstrations of BCARS imaging for histopathology, cell, and whole organism imaging will be presented as well. |
14:05 |
Nonlinear Optical Spectroscopy and Imaging
Main Author: Zhiwen Liu Organization: Penn State, United States Nonlinear spectroscopy and imaging modalities enabled by second and third order nonlinear optical processes will be presented. Second order nonlinearity, in particular that of two-dimensional transition metal dichalcogenides (e.g. WS2 and MoS2) which possess extraordinary effective bulk-like nonlinear susceptibility (several nm/V), can potentially provide new second harmonic imaging probes. Vibrational response, probed by using sum frequency generation and coherent anti-Stokes Raman scattering, provides a means for chemical contrast, while coherent holographic detection captures both the amplitude and the phase of a complex signal field to enable label-free three-dimensional imaging. Materials characterization and underlying imaging principles will be presented. Potential applications will also be discussed. |
14:30 |
Ultrafast Pulse Burst Ablation of Cartilage for Applications in Orthopaedic Surgery
Main Author: Margarete Akens Organization: University Health Network, Canada A femtosecond burst-mode fiber laser system was investigated as a tool to prepare cartilage graft beds. The laser's ability to ablate articular cartilage and its effect on laser-tissue interaction was examined using confocal microscopy, optical coherence tomography, and histology. Large volumes were ablated while delivering minimal collateral damage to the tissue. Delivery of the light with a fiber would allow for arthroscopic surgery preventing the need for open knee surgery. |