Session Overview |
Thursday, May 30 |
10:40 |
Numerical and experimental study of spectrum shuttle for generating GHz burst pulses without sacrificing spectral ranges in advanced ultrafast imaging
* Keitaro Shimada, The University of Tokyo, Japan Ayumu Ishijima, The University of Tokyo Takao Saiki, The University of Tokyo Ichiro Sakuma, The University of Tokyo Yuki Inada, Saitama University Keiichi Nakagawa, The University of Tokyo For burst pulse generation in ultrafast photography, the significant loss of spectral ranges remains a persistent challenge. To address this, we present a spectrum shuttle, an optical technique that produces spatially nondispersive and spectrally separated GHz burst pulse. We demonstrated the spectrum shuttle effectively preserved these ranges through simulations and experiments. The utility of the generated burst pulses was validated in two-color single-shot imaging of laser ablation dynamics with 250 ps frame intervals in 800-nm and 400-nm wavelength bands. |
10:55 |
Optical coherence tomography angiography and its biomedical applications
* Ruikang Wang, University of Washington, United States of America Optical coherence tomography (OCT) is a new medical imaging modality in which the coherent interference of a wide spectrum light source is used to create a high resolution (micron-scale) subsurface image of tissue microstructure. Recently, we have supplemented the microstructural OCT images with additional contrast mechanisms such as blood flow imaging using static and motion contrast, which provide us the ability to perform label-free optical microangiography (OMAG) of microcirculatory tissue beds [1,2]. The ability to visualize tissue blood flow at the microcirculation level is important in a variety of biomedical applications, some of which (along with the OCT basics and the enabling technologies) will be highlighted in this talk. Examples using OMAG to delineate the dynamic blood perfusion, down to capillary level resolution, within living tissue will be given, including cerebral blood flow in small animals, and retinal blood flow in humans. |
11:30 |
Multispectral Imaging of Fluorescent-Tagged Leaves
* Emma Abbey, University of Victoria, Canada Travis Ferguson, University of Victoria Hans-Peter Loock, University of Victoria We present an application of our home-built multispectral fluorescence imaging system for monitoring degradation in Arabidopsis leaves. Our system uses a Hadamard-multiplexed programmable light source based on a digital micromirror array, and a multispectral snapshot camera for detection of fluorescent compounds. |
11:45 |
Towards the diagnostic application of ultraweak photon emission: Monitoring magnetic field effects and oxidative stress in green leaves
* Vishnu Seshan, University of Calgary, Canada Lujaina Eldelebshany, University of Calgary Vahid Salari, University of Calgary Ayesha Iqbal, University of Calgary Lana Frankle, University of Calgary Daniel Oblak, University of Calgary This study explores the use of delayed luminescence (DL) and ultraweak photon emission (UPE) as indicators of reactive oxygen species (ROS) in biological systems. Employing an electron multiplying charge coupled device (EMCCD) camera and photomultiplier tube (PMT), the research investigates spatiotemporal changes in photon emission under weak magnetic fields. The study extends to assessing oxidative stress in green leaves caused by various perturbations, employing machine learning techniques for classification. Preliminary experiments demonstrate promising results, including achieving up to 94% accuracy in leaf perturbation classification using neural networks. Future work aims to broaden the study's scope and deepen mechanistic understanding of the underlying biochemistry. |